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KMID : 0614620040440010025
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Korean Journal of Gastroenterology
2004 Volume.44 No. 1 p.25 ~ p.33
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Alimentary Tract : The Role of Interleukin-1β Gene Polymorphism in the Gastric Carcinogenesis
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Kang Won-Kyung
Park Won-Sang
Chin Hyung-Min
Park Cho-Hyun
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Abstract
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Background/Aims: This study was aimed to investigate the polymorphism of interleukin-1β (IL-1B) and IL-1 receptor antagonist (IL-1RN) gene and the relationship between genotypes and development of gastric adenocarcinoma in Korean, and to investigate the role of Helicobactor pylori (H. pylori) infection.
Methods: The study population comprised of 258 patients with gastric adenocarcinoma. They were classified according to Lauren´s classification and the status of H. pylori infection. Genomic DNA was extracted from the gastric tissue.
As a control, genomic DNA from peripheral lymphocyte of 100 healthy individuals was used. The amplified products of -511 bp and -31 bp fragments in the IL-1B by PCR were digested by restriction enzyme and separated for RFLP. Variable number tandem repeats were amplified and subjected to RFLP of IL-1RN.
Results: There was no significant difference in the genotype of IL-1B-511T and IL-1B-31C between the adenocarcinoma group and the control group. IL-1RN allele 1 homozygote in the intestinal type showed high frequency of 91.7% (p=0.007). In the H. pylori-positive group of the adenocarcinoma, the frequency of IL-1B-31C was significantly higher than that of H. pylori-negative group (p=0.045).
Conclusions: The single nucleotide polymorphism of IL-1B-31C may contribute to the development of the gastric enocarcinoma in the H. pylori-positive population.
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KEYWORD
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Stomach cancer, Polymorphism, single nucleotide, Interleukin-1β, Helicobactor pylori
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